Nanostructure-Initiator Mass Spectrometry Highlighted in Science and Nature


Nanoscience and mass spectrometry have been combined to produce a new, high throughput method to determine the functions of biologically active molecules, e.g., identification of particular metabolites as evidence that a particular cellular energy pathway is active. Developed by Genomics:GTL scientists Gary Siuzdak and Trent Northern of the Scripps Institute and the Lawrence Berkeley National Laboratory, this new research tool is highlighted in a Perspective article in the September 19 issue of Science. The underlying technology called nanostructure-initiator mass spectrometry (NMIS) is also spotlighted in a Technology Feature article in the October 2 issue of Nature. The technique, called Nimzyme analysis, involves the tagging of the biological molecule(s) to be tested with a fluorous tag that gets embedded in the perfluorosiloxane “initiatior” compound that fill nanosized holes on a specially-prepared surface. This methods allows an array of many embedded molecules of interest to be exposed to a solution containing a specific enzyme or mixtures of other biological molecules of interest followed by a laser pulse or beam of ionizing energy. The irradiation vaporizes the material in the nanoholes, releasing the biological molecules for rapid analysis by mass spectrometer to determine any molecular changes in the florous tagged molecules. The approach has the potential for what the Science Perspective article characterized as “high throughput bioprospecting applications.”


Perspective: D. Curran, Science, 321, 1645 (based on article, T. R. Northen et al., Proc. Nat. Acad. Sci. U.S.A. 105, 3678 (2008)).

Technology Feature: N. Blow, Nature 455, 697-700