Methyl Ketone Production by Pseudomonas putida Is Enhanced by Plant-Derived Amino Acids

The Science

Methyl ketones are a promising diesel blendstock and have been produced by multiple microbial platforms. Test use of plant-derived aromatics and amino acids to improve the efficiency of overall biomass conversion to methyl ketones.

The Impact

  • Engineered P. putida produced methyl ketones from glucose and lignin-related aromatics.
  • Methyl ketone production by P. putida increased with plant hydrolysates as substrates in comparison to sugar controls.
  • Metabolomics and proteomics demonstrated that the increased production was due to plant-derived amino acids.
  • Inclusion of a defined amino acid mixture increased methyl ketone production 9-fold relative to a sugar-only control.


  • Engineered Pseudomonas putida to produce methyl ketones by introducing a truncated beta-oxidation pathway.
  • Tested the strain on pure substrates and plant hydrolysates containing significant amounts of plant-derived aromatics.


Dong et al. “Methyl ketone production by Pseudomonas putida is enhanced by plant-derived amino acids,” Biotechnology and Bioengineering 116(8), 1909–1922 (2019). [DOI:10.1002/bit.26995]