Magic Pools: Parallel Assessment of Transposon Delivery Vectors in Bacteria

The Science

Streamlined approaches for testing hundreds to thousands of genetic systems in parallel against a target bacterium.

The Impact

• Uses a “parts-based” synthetic biology cloning strategy.
• Uses random DNA barcode sequences to track the efficacy of many transposon vectors in parallel.
• Successfully adapted the strategy to 5 different genera of bacteria, including 3 from the phylum Bacteroidetes.

Summary

• Uses a “parts-based” synthetic biology cloning strategy.
• Uses random DNA barcode sequences to track the efficacy of many transposon vectors in parallel.
• Successfully adapted the strategy to 5 different genera of bacteria, including 3 from the phylum Bacteroidetes.

References

Liu, H. et al. “Magic pools: Parallel assessment of transposon delivery vectors in bacteria.” mSystems 3(1) e00143-17 (2017). [DOI:10.1128/mSystems.00143-17]